LS-B16500

antibody from LSBio

Targeting: TUBB M40, MGC16435, OK/SW-cl.56, Tubb5

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B16500

Provider

LSBio

Product name

IHC-plus™ TUBB / Beta Tubulin Antibody (aa238-429) LS-B16500

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Hamster

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A:293T, B: H1299. 10% SDS PAGE. TUBB / Beta Tubulin antibody diluted at 1:3000.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. TUBB / Beta Tubulin antibody diluted at 1:5000.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (whole cell lysate). A:293T 20 ug, B:293T 10 ug, C:293T 5 ug. 10% SDS PAGE. TUBB / Beta Tubulin antibody diluted at 1:10000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A:293T, B: H1299. 10% SDS PAGE. TUBB / Beta Tubulin antibody diluted at 1:3000.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using beta Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using beta Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.