Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [5]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32008 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta-5 Tubulin Recombinant Rabbit Monoclonal Antibody (SR25-04)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SR25-04
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of beta-5 Tubulin in different lysates using a Monoclonal antibody (Product #MA5-32008) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: PC12.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of beta-5 Tubulin in different lysates using a Monoclonal antibody (Product #MA5-32008) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: PC12.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of beta-5 Tubulin in Hela cells using a beta-5 Tubulin Monoclonal antibody (Product # MA5-32008) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of beta-5 Tubulin in CRC cells using a beta-5 Tubulin Monoclonal antibody (Product # MA5-32008) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of beta-5 Tubulin in N2A cells using a beta-5 Tubulin Monoclonal antibody (Product # MA5-32008) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of beta-5 Tubulin in PC12 cells using a beta-5 Tubulin Monoclonal antibody (Product # MA5-32008) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of beta-5 Tubulin in SH-SY-5Y cells using a beta-5 Tubulin Monoclonal antibody (Product # MA5-32008) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of beta-5 Tubulin of paraffin-embedded Mouse brain tissue using a beta-5-Tubulin Monoclonal antibody (Product #MA5-32008). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of beta-5 Tubulin of paraffin-embedded Mouse kidney tissue using a beta-5-Tubulin Monoclonal antibody (Product #MA5-32008). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of beta-5 Tubulin of paraffin-embedded Human colon cancer tissue using a beta-5-Tubulin Monoclonal antibody (Product #MA5-32008). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of beta-5 Tubulin of paraffin-embedded Human kidney tissue using a beta-5-Tubulin Monoclonal antibody (Product #MA5-32008). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of beta-5 Tubulin of paraffin-embedded Mouse colon tissue using a beta-5-Tubulin Monoclonal antibody (Product #MA5-32008). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of beta-5 Tubulin in NIH/3T3 cells using a beta-5 Tubulin Monoclonal Antibody (Product # MA5-32008) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody..