ARG63199

antibody from Arigo

Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

ARG63199

Provider

Arigo

Product name

anti-FOXP3 antibody

Antibody type

Polyclonal

Antigen

SQRPSRCSNPTPGP

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: CD25-sorted (Treg) human blood cells gathered by cytospin stained with ARG63199 anti-FOXP3 antibody and detected by FITC (A) and in phase contrast (B).

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: paraffin embedded Human Tonsil. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63199 anti-FOXP3 antibody at 1 µg/ml dilution followed by HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.