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LearnSTJ70552
antibody from St John's Laboratory
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- STJ70552 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-FOXP3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
KRSQRPNKCSNP- Epitope
- C-term
- Isotype
- IgG
- Vial size
- 100μg,,
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70552 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- In paraffin embedded Mouse Spleen shows patches of stained cells in the Germinal Centre. Recommended concentration, 2-4µg/ml.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
