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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 24032-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ANKRD50 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TAMRVAAKN GHSQIIKLLE KYGASSLNGC SPSPVHTMEQ KPLQSLSSKV QSLTIKSNSS GSTGGGDMQP SLRGLPNGPT HAFSSPSESP DSTVDRQKSS LSNNSLKSSK NSSLRTTSST ATAQTVPIDS FHNLSFTEQI QQHSLPRSRS RQSIVSPSST TQSLGQSHNS PSSEFEWSQV KPSLKSTKAS KGGKSENSAK SGSAGKKAKQ SNSSQPKVLE YEMTQFDRRG PIAKSGTAAP PKQMPAESQC KIMIPSAQQE IGRSQQQFLI HQQSGEQKKR NGIMTNPNYH LQSNQVFLGR VSVPRTMQDR GHQEVLEGYP SSETELSLKQ ALKLQIEGSD PSFNYKKETP L (1080-1429 aa encoded by BC024725)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 24032-1-AP ( ANKRD50 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 24032-1-AP (ANKRD50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ANKRD50 (IP:24032-1-AP, 5ug; Detection:24032-1-AP 1:300) with HEK-293 cells lysate 4000ug.
