MA5-29212

antibody from Invitrogen Antibodies

Targeting: DCUN1D2 C13orf17, FLJ10704, FLJ20092

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29212

Provider

Invitrogen Antibodies

Product name

DCUN1D2 Recombinant Rabbit Monoclonal Antibody (1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

1

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCUN1D2 in Lane A: HCT116 Whole Cell Lysate (30 µg). Samples were probed using a DCUN1D2 Monoclonal Antibody (Product # MA5-29212) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:30 kDa. Observed band size:30 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DCUN1D2 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29212) at 1:500 dilution. Lane A: HCT116 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DCUN1D2 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg A549 Whole Cell Lysate, Lane C: 0.5 mg HuT78 Whole Cell Lysate 2 µL with DCUN1D2 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29212) and 15 µL of 50 % Protein G agarose. Primary antibody: DCUN1D2 Recombinant Rabbit Monoclonal Antibody (1), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.