PA5-118316

antibody from Invitrogen Antibodies

Targeting: TMEM255B FAM70B, MGC20579

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118316

Provider

Invitrogen Antibodies

Product name

TMEM255B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Specificity: Human TMEM255B

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TMEM255B Polyclonal Antibody (Product # PA5-118316) at 1:500 dilution. Lane A: U87MG Whole Cell Lysate, Lane B: PC3 Whole Cell Lysate, Lane C: RPMI-8226 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 33 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of FAM70B in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with TMEM255B Polyclonal Antibody (Product # PA5-118316, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TMEM255B Immunoprecipitation using: Lane A: 0.5 mg U87MG Whole Cell Lysate 4 µL with TMEM255B Polyclonal Antibody (Product # PA5-118316) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: TMEM255B Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 32 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TMEM255B Immunoprecipitation using: Lane A: 0.5 mg U87MG Whole Cell Lysate 4 µL with TMEM255B Polyclonal Antibody (Product # PA5-118316) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: TMEM255B Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 32 kDa.