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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB1728 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Semaphorin 3C Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Semaphorin 3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 6B, 6C, 6D, recombinant mouse Semaphorin 3A, 3B, 3E, 3F, 6A, or 7A is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q62181- Isotype
- IgG
- Antibody clone number
- 238835
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The cleavage of semaphorin 3C induced by ADAMTS1 promotes cell migration.
Esselens C, Malapeira J, Colomé N, Casal C, RodrÃguez-Manzaneque JC, Canals F, Arribas J
The Journal of biological chemistry 2010 Jan 22;285(4):2463-73
The Journal of biological chemistry 2010 Jan 22;285(4):2463-73
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Semaphorin 3C in MCF-7 Human Cell Line. Semaphorin 3C was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Mouse Semaphorin 3C Monoclonal Antibody (Catalog # MAB1728) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Semaphorin 3C in Mouse Embryo. Semaphorin 3C was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Human/Mouse Semaphorin 3C Monoclonal Antibody (Catalog # MAB1728) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
