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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB10289 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10289, RRID:AB_11189672
- Product name
- OTX2 monoclonal antibody, clone 1H12G8B2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant OTX2.
- Isotype
- IgG
- Antibody clone number
- 1H12G8B2
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) against recombinant human OTX2 protein.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of U251 cells using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon tissue (A), colon cancer tissue (B), stomach tissue (C) and brain tissue (D) using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
