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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF988 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal JAM-B/VE-JAM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse JAM-B in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human JAM-B is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF.
Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation.
Su RW, Jia B, Ni H, Lei W, Yue SL, Feng XH, Deng WB, Liu JL, Zhao ZA, Wang TS, Yang ZM
PloS one 2012;7(4):e34325
PloS one 2012;7(4):e34325
Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation.
Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schön MP, Kaufmann R, Boehncke WH, Podda M
The Journal of investigative dermatology 2005 Nov;125(5):969-76
The Journal of investigative dermatology 2005 Nov;125(5):969-76
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Adhesion Mediated by JAM-B/VE-JAM and Neutralization by Mouse JAM-B/VE-JAM Antibody. Recombinant Mouse JAM-B/VE-JAM Fc Chimera (Catalog # 988-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse JAM-B/VE-JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse JAM-B/VE-JAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF988). The ND50 is typically 0.6-3.0 µg/mL.
