H00058494-M01

antibody from Abnova Corporation

Targeting: JAM2 C21orf43, CD322, JAM-B, JAMB, VE-JAM

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00058494-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00058494-M01, RRID:AB_426048

Product name

JAM2 monoclonal antibody (M01), clone 1G4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant JAM2.

Antigen sequence

FSAPKDQQVVTAVEYQEAILACKTPKKTVSSRLEW
KKLGRSVSFVYYQQTLQGDFKNRAEMIDFNIRIKN
VTRSDAGKYRCEVSAPSEQGQNLEEDTVTLEVLVA
PAVPSCEVPSSALSGTVVELRCQDKEGNPAPEYTW
FKDGIRLLENPRLGSQSTNSSYTMNTKTGTLQFNT
VSKLDTGEYSCEARNSVGYRRCPGKRMQVDDLNIS
GIIAAVVVVALVISVCGLGVCYAQRKGYFSKETSF
QKSNSSSKATTMSENDFKHTKSFII


Isotype

IgG

Antibody clone number

1G4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of JAM2 expression in transfected 293T cell line by JAM2 monoclonal antibody (M01), clone 1G4.Lane 1: JAM2 transfected lysate (Predicted MW: 33.2 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

JAM2 monoclonal antibody (M01), clone 1G4. Western Blot analysis of JAM2 expression in HL-60.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

JAM2 monoclonal antibody (M01), clone 1G4. Western Blot analysis of JAM2 expression in human ovarian cancer.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged JAM2 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to JAM2 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol