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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG54011 - Provider product page
- Provider
- Arigo
- Product name
- anti-SMC1A antibody (N-term)
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human SMC1A(N-terminus) protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8E6-A4-F3
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: SMC1A (N-terminus) in HeLaNE, Jurkat and A431 cell lysates stained with SMC1A antibody [8E6-A4-F3]inal (ARG54011) (diluted in 1: 1000). Predicted band size: 143KDa. Observed band size: 143KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human colon stained with SMC1A antibody [8E6-A4-F3], N-terminal (ARG54011) (diluted in 1/500-1/1000).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with SMC1A antibody [8E6-A4-F3], N-terminal (ARG54011) (red, 1/100), followed by FITC-conjugated goat mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
