A700-018
antibody from Invitrogen Antibodies
Targeting: SMC1A
DXS423E, KIAA0178, SB1.8, SMC1L1, Smcb
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- A700-018 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMC1 Recombinant Rabbit Monoclonal Antibody (BL-205-2G8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BL-205-2G8
- Vial size
- 100 µL
- Concentration
- 50 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SMC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMC1 by WB of immunoprecipitates from 293T lysate. Antibodies: Rabbit anti-SMC1 recombinant monoclonal {BL-205-2G8] (A700-018) and rabbit anti-SMC1 (A300-055A). Secondary: ReliaBLOT® reagents (WB120).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SMC1 by WB in HeLa, 293T, MCF7, HepG2, A549, SW620, SK-MEL28 (SK), Jurkat, TCMK, NIH3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (A700-018). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMC1 in FFPE NCI-H226 cells by ICC.Antibody:Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2).Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P).Substrate:DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMC1 in FFPE HCT-116 cells by ICC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (A700-018). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse SMC1 in FFPE renal cell carcinoma by IHC.Antibody:Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2).Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P).Substrate:DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMC1 in FFPE ovarian carcinoma by IHC.Antibody:Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2).Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P).Substrate:DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMC1 in FFPE breast carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMC1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (A700-018). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMC1 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (Product # A700-018; lot 1) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG (Product # A120-201D5).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2) used for IP at 20 µL/mg lysate. SMC1 was also immunoprecipitated by a previous lot of this antibody (lot A700-018-1) and rabbit anti-SMC1 antibody (Product # A300-055A). For blotting immunoprecipitated SMC1 (Product # A700-018) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.