A700-018

antibody from Invitrogen Antibodies

Targeting: SMC1A DXS423E, KIAA0178, SB1.8, SMC1L1, Smcb

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-018

Provider

Invitrogen Antibodies

Product name

SMC1 Recombinant Rabbit Monoclonal Antibody (BL-205-2G8)

Antibody type

Monoclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Antibody clone number

BL-205-2G8

Concentration

50 µg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse SMC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC1 by WB of immunoprecipitates from 293T lysate. Antibodies: Rabbit anti-SMC1 recombinant monoclonal {BL-205-2G8] (A700-018) and rabbit anti-SMC1 (A300-055A). Secondary: ReliaBLOT® reagents (WB120).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse SMC1 by WB in HeLa, 293T, MCF7, HepG2, A549, SW620, SK-MEL28 (SK), Jurkat, TCMK, NIH3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (A700-018). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC1 in FFPE NCI-H226 cells by ICC.Antibody:Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2).Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P).Substrate:DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC1 in FFPE HCT-116 cells by ICC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (A700-018). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse SMC1 in FFPE renal cell carcinoma by IHC.Antibody:Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2).Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P).Substrate:DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC1 in FFPE ovarian carcinoma by IHC.Antibody:Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2).Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P).Substrate:DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC1 in FFPE breast carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (A700-018). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC1 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (Product # A700-018; lot 1) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG (Product # A120-201D5).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (Product # A700-018 lot 2) used for IP at 20 µL/mg lysate. SMC1 was also immunoprecipitated by a previous lot of this antibody (lot A700-018-1) and rabbit anti-SMC1 antibody (Product # A300-055A). For blotting immunoprecipitated SMC1 (Product # A700-018) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.