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- Antigen structure
- References [9]
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- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NB100-206 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-206, RRID:AB_10002504
- Product name
- Rabbit Polyclonal SMC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation.
A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity.
SMRT compounds abrogate cellular phenotypes of ataxia telangiectasia in neural derivatives of patient-specific hiPSCs.
Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum.
The foci of DNA double strand break-recognition proteins localize with gammaH2AX after heat treatment.
The foci of DNA double strand break-recognition proteins localize with gammaH2AX after heat treatment.
ATM is down-regulated by N-Myc-regulated microRNA-421.
Rapid flow cytometry-based structural maintenance of chromosomes 1 (SMC1) phosphorylation assay for identification of ataxia-telangiectasia homozygotes and heterozygotes.
Phosphorylation of Chk1 by ATR is antagonized by a Chk1-regulated protein phosphatase 2A circuit.
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD
The EMBO journal 2015 May 12;34(10):1399-416
The EMBO journal 2015 May 12;34(10):1399-416
A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity.
Nakamura K, Fike F, Haghayegh S, Saunders-Pullman R, Dawson AJ, Dörk T, Gatti RA
Molecular genetics & genomic medicine 2014 Jul;2(4):332-40
Molecular genetics & genomic medicine 2014 Jul;2(4):332-40
SMRT compounds abrogate cellular phenotypes of ataxia telangiectasia in neural derivatives of patient-specific hiPSCs.
Lee P, Martin NT, Nakamura K, Azghadi S, Amiri M, Ben-David U, Perlman S, Gatti RA, Hu H, Lowry WE
Nature communications 2013;4:1824
Nature communications 2013;4:1824
Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum.
Du L, Kayali R, Bertoni C, Fike F, Hu H, Iversen PL, Gatti RA
Human molecular genetics 2011 Aug 15;20(16):3151-60
Human molecular genetics 2011 Aug 15;20(16):3151-60
The foci of DNA double strand break-recognition proteins localize with gammaH2AX after heat treatment.
Takahashi A, Mori E, Ohnishi T
Journal of radiation research 2010;51(1):91-5
Journal of radiation research 2010;51(1):91-5
The foci of DNA double strand break-recognition proteins localize with gammaH2AX after heat treatment.
Takahashi A, Mori E, Ohnishi T
Journal of radiation research 2010;51(1):91-5
Journal of radiation research 2010;51(1):91-5
ATM is down-regulated by N-Myc-regulated microRNA-421.
Hu H, Du L, Nagabayashi G, Seeger RC, Gatti RA
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 26;107(4):1506-11
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 26;107(4):1506-11
Rapid flow cytometry-based structural maintenance of chromosomes 1 (SMC1) phosphorylation assay for identification of ataxia-telangiectasia homozygotes and heterozygotes.
Nahas SA, Butch AW, Du L, Gatti RA
Clinical chemistry 2009 Mar;55(3):463-72
Clinical chemistry 2009 Mar;55(3):463-72
Phosphorylation of Chk1 by ATR is antagonized by a Chk1-regulated protein phosphatase 2A circuit.
Leung-Pineda V, Ryan CE, Piwnica-Worms H
Molecular and cellular biology 2006 Oct;26(20):7529-38
Molecular and cellular biology 2006 Oct;26(20):7529-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from HeLa cells that were treated with NCS (+; 200 ng/ml; 1h) or mock treated (-). Antibody used at 0.1 ug/ml. For detection of total SMC1, rabbit anti-SMC1 antibody was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Analysis if human Phospho SMC1 (Ser966). Sample: Whole cell extracts from HeLa cells (30 ug/lane) that were irradiated with 10 Gy of ionizing radiation.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SMC1 [p Ser966] Antibody [NB100-206] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human testicular seminoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human breast carcinoma. Antibody: Affinity purified rabbit antiPhospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
