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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB9966-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RTTN Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human RTTN in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q86VV8- Isotype
- IgG
- Antibody clone number
- 2311A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RTTN by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RTTN at approximately 248 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RTTN in HDLM-2 Human Cell Line. RTTN was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RTTN in Human Stomach. RTTN was detected in immersion fixed paraffin-embedded sections of human stomach using Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
