AMAb90803

antibody from Atlas Antibodies

Targeting: PGAM5 BXLBv68, MGC5352

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb90803

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90803, RRID:AB_2665674

Product name

Anti-PGAM5

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

NWDRREPLSLINVRKRNVESGEEELASKLDHYKAK
ATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAEL
TGLRLASLGLKFNKIV


Epitope

Binds to an epitope located within the peptide sequence NWDRREPLSLINVRK as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL0624

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PGAM5 antibody. Remaining relative intensity is presented. Loading control: Anti-PARP1.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa]Lane 2: Human cell line RT-4

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows either strong or moderate cytoplasmic immunoreactivity in subsets of renal tubules.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows moderate cytoplasmic immunoreactivity in hepatocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in the seminiferous ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human uterus shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong immunoreactivity in germinal center cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohisochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.