PAB27852

antibody from Abnova Corporation

Targeting: PGAM5 BXLBv68, MGC5352

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27852

Provider

Abnova Corporation

Product name

PGAM5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PGAM5.

Antigen sequence

NWDRREPLSLINVRKRNVESGEEELASKLDHYKAK
ATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAEL
TGLRLASLGLKFNKIV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with PGAM5 polyclonal antibody (Cat # PAB27852) at 1:500-1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line MCF-7 with PGAM5 polyclonal antibody (Cat # PAB27852) at 1-4 ug/mL dilution shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human uterus, pre-menopause with PGAM5 polyclonal antibody (Cat # PAB27852) shows strong cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)