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Flow cytometry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Other assay [1]
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- Product number
- MAB1849-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NKp30/NCR3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NKp30/NCR3 on cell transfectants and peripheral blood NK cells.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 210845
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and γδ T cells.
Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands.
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
A novel binding assay to assess specificity of monoclonal antibodies.
Djaoud Z, Guethlein LA, Horowitz A, Azzi T, Nemat-Gorgani N, Olive D, Nadal D, Norman PJ, Münz C, Parham P
The Journal of experimental medicine 2017 Jun 5;214(6):1827-1841
The Journal of experimental medicine 2017 Jun 5;214(6):1827-1841
Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands.
Herrmann J, Berberich H, Hartmann J, Beyer S, Davies K, Koch J
The Journal of biological chemistry 2014 Jan 10;289(2):765-77
The Journal of biological chemistry 2014 Jan 10;289(2):765-77
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD Jr, Barlogie B, van Rhee F
Blood 2008 Feb 1;111(3):1309-17
Blood 2008 Feb 1;111(3):1309-17
A novel binding assay to assess specificity of monoclonal antibodies.
Warren HS, Bettadapura J
Journal of immunological methods 2005 Oct 20;305(1):33-8
Journal of immunological methods 2005 Oct 20;305(1):33-8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Human NKp30 Antibody Induces IFN-gamma Secretion in NK-92 Cells. Human NKp30 Monoclonal Antibody induces IFN-gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Quantikine Human IFN-gamma ELISA Kit (Catalog # DIF50). The ED50 for this effect is typically 0.2-1.2 μg/mL.
