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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-78830 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal NCKAP5L Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 100 ul
- Concentration
- 0.04 mg/ml
- Storage
- Store at 2 - 8 C 1 year from date of receipt
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCKAP5L Antibody [NBP2-78830] - Detection of human and mouse NCKAP5L by western blot. Samples: Whole cell lysate (10 ug) from K-562, Hep-G2, LNCaP, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCKAP5L antibody NBP2-78830 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NCKAP5L Antibody [NBP2-78830] - Detection of human NCKAP5L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP5L antibody NBP2-78830 used for IP at 100 ul per reaction. NCKAP5L was also immunoprecipitated by another rabbit anti-NCKAP5L antibody. For blotting immunoprecipitated NCKAP5L, NBP2-78830 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
