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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- 23795-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLXNB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LQPLPPTAF TPNGTYLQHL ARDPTSGTLY LGATNFLFQL SPGLQLEATV STGPVLDSRD CLPPVMPDEC PQAQPTNNPN QLLLVSPGAL VVCGSVHQGV CEQRRLGQLE QLLLRPERPG DTQYVAANDP AVSTVGLVAQ GLAGEPLLFV GRGYTSRGVG GGIPPITTRA LWPPDPQAAF SYEETAKLAV GRLSEYSHHF VSAFARGASA YFLFLRRDLQ AQSRAFRAYV SRVCLRDQHY YSYVELPLAC EGGRYGLIQA AAVATSREVA HGEVLFAAFS SAAPPTVGRP PSAAAGASGA SALCAFPLDE VDRLANRTRD ACYTREGRAE DGTEVAYIEY DVNSDCAQLP V (20-369 aa encoded by BC146793)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 23795-1-AP ( PLXNB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 23795-1-AP ( PLXNB1 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 23795-1-AP ( PLXNB1 antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 SH-SY5Y cells were stained with 0.2ug PLXNB1 antibody (23795-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
