PAB28620

antibody from Abnova Corporation

Targeting: POLA1 NSX, p180, POLA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28620

Provider

Abnova Corporation

Product name

POLA1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant POLA1.

Antigen sequence

VDLEPMAAKAWDKESEPAEEVKQEADSGKGTVSYL
GSFLPDVSCWDIDQEGDSSFSVQEVQVDSSHLPLV
KGADEEQVFHFYWLDAYEDQYNQPGVVFLFGKVWI
ESAETHVSCCVMVK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp, Lane 3: Human cell line A-431 with POLA1 polyclonal antibody (Cat # PAB28620) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with POLA1 polyclonal antibody (Cat # PAB28620) at 1-4 ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with POLA1 polyclonal antibody (Cat # PAB28620) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)