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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 16513-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXXC5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MMGGESADK ATAAAAAASL LANGHDLAAA MAVDKSNPTS KHKSGAVASL LSKAERATEL AAEGQLTLQQ FAQSTEMLKR VVQEHLPLMS EAGAGLPDME AVAGAEALNG QSDFPYLGAF PINPGLFIMT PAGVFLAESA LHMAGLAEYP MQGELASAIS SGKKKRKRCG MCAPCRRRIN CEQCSSCRNR KTGHQICKFR KCEELKKKPS AALEKVMLPT GAAFRWFQ (96-322 aa encoded by BC017439 )
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16513-1-AP ( CXXC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 16513-1-AP (CXXC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16513-1-AP (CXXC5 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CXXC5 (IP: 16513-1-AP, 4 µg; Detection: 16513-1-AP 1:300) with SH-SY5Y cells lysate 3400 µg.
