25589-1-AP

antibody from Invitrogen Antibodies

Targeting: SMCHD1 FSHD2, KIAA0650

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25589-1-AP

Provider

Invitrogen Antibodies

Product name

SMCHD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LLTFPDNVE HCETVFGMLL GDTIILDNLD AANHYRKEVV KITHCPTLLT RDGDRIRSNG KFGGLQNKAP PMDKLRGMVF GAPVPKQCLI LGEQIDLLQQ YRSAVCKLDS VNKDLNSQLE YLRTPDMRKK KQELDEHEKN LKLIEEKLGM TPIRKCNDSL RHSPKVETTD CPVPPKRMRR EATRQNRIIT KTDV (1813-2005 aa encoded by BC035774)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 25589-1-AP ( SMCHD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 25589-1-AP (SMCHD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).