Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-82978 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82978, RRID:AB_11020741
- Product name
- Rabbit Polyclonal SMCHD1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SMCHD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Digenic inheritance of an SMCHD1 mutation and an FSHD-permissive D4Z4 allele causes facioscapulohumeral muscular dystrophy type 2.
Lemmers RJ, Tawil R, Petek LM, Balog J, Block GJ, Santen GW, Amell AM, van der Vliet PJ, Almomani R, Straasheijm KR, Krom YD, Klooster R, Sun Y, den Dunnen JT, Helmer Q, Donlin-Smith CM, Padberg GW, van Engelen BG, de Greef JC, Aartsma-Rus AM, Frants RR, de Visser M, Desnuelle C, Sacconi S, Filippova GN, Bakker B, Bamshad MJ, Tapscott SJ, Miller DG, van der Maarel SM
Nature genetics 2012 Dec;44(12):1370-4
Nature genetics 2012 Dec;44(12):1370-4
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SMCHD1 Antibody [NBP1-82978] - Simple Western lane view shows a specific band for SMCHD1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SMCHD1 Antibody [NBP1-82978] - Electropherogram image(s) of corresponding Simple Western lane view. SMCHD1 antibody was used at 1:40 dilution on RT-4 lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMCHD1 Antibody [NBP1-82978] - Analysis in human cell line HL-60.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMCHD1 Antibody [NBP1-82978] - Analysis in human cell lines U2OS and MCF-7 using Anti-SMCHD1 antibody. Corresponding SMCHD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-82978] - Staining of human duodenum shows nucellar positivity in glandular cells.