PA5-46624

antibody from Invitrogen Antibodies

Targeting: MIEF2 MGC23130, MiD49, SMCR7

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46624

Provider

Invitrogen Antibodies

Product name

SMCR7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: FSQKRGKRRS DEGLGSMVDF LLANARLVLG VGGAAVLGIA TLAVKRFIDR

Concentration

0.5 mg/mL

References

Submitted references

HPV/E7 induces chemotherapy-mediated tumor suppression by ceramide-dependent mitophagy.

Thomas RJ, Oleinik N, Panneer Selvam S, Vaena SG, Dany M, Nganga RN, Depalma R, Baron KD, Kim J, Szulc ZM, Ogretmen B
EMBO molecular medicine 2017 Aug;9(8):1030-1051

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human HT1080 cell lysate using an anti-SMCR7 polyclonal antibody (Product # PA5-46624).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9 E2F5 enhances Drp1 translocation to mitochondria and association with MFF to induce HPV-E7/ceramide-dependent mitophagy Effects of stable knockdown of E2F5 using shRNA on DRP1-MFF association with/without C 18 -pyr-cer (10 muM, 1 h) were measured by PLA (scale bars represent 100 mum) using anti-DRP1 and anti-MFF antibodies compared to Scr-shRNA-transfected UM-SCC-47 controls. PLA fluorescence images were quantified using the PLA software as described by the manufacturer. Data are means +- SD from three independent experiments, analyzed by unpaired Student's t -test ( n = 3, * P = 0.0042). HPV(-) UM-SCC-22A cells transiently transfected with exogenous E2F5 or empty vector (vec) were used to measure the association between Drp1 and MFF in the absence/presence of C 18 -pyr-cer (10 muM, 1 h) by PLA (scale bars represent 100 mum) using anti-DRP1 and anti-MFF antibodies. PLA fluorescence images were quantified using the PLA software as described by the manufacturer. Data are means +- SD from three independent experiments, analyzed by unpaired Student's t -test ( n = 3, * P = 0.0051). Effects of C 18 -pyr-cer (10 muM, 1 h) on Drp1-MFF interaction in the absence/presence of E2F5 knockdown using shRNA (versus Scr-shRNA) were measured by immunoprecipitation followed by Western blotting using anti-Drp1 and anti-MFF antibodies (right panel). Equal immunoprecipitation of Drp1 or MFF was confirmed by Western blotting (left panel, input). Blots represent three independent studies