Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-99984 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMCR7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Clueless/CLUH regulates mitochondrial fission by promoting recruitment of Drp1 to mitochondria.
Yang H, Sibilla C, Liu R, Yun J, Hay BA, Blackstone C, Chan DC, Harvey RJ, Guo M
Nature communications 2022 Mar 24;13(1):1582
Nature communications 2022 Mar 24;13(1):1582
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMCR7 in HT-1080 cells. Samples were incubated with SMCR7 polyclonal antibody (Product # PA5-99984).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMCR7 in various samples (Lane 1: 3T3 treated with blocking peptide, Lane 2: 3T3, Lane 3: COS-7, Lane 4: MCF7). Samples were incubated with SMCR7 polyclonal antibody (Product # PA5-99984).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SMCR7 in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with SMCR7 polyclonal antibody (Product # PA5-99984) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded SMCR7 in mouse muscle tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with SMCR7 polyclonal antibody (Product # PA5-99984) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 8 CLUH controls MiD49 and Mff protein levels through translational regulation in mammalian cells. a - d Immunofluorescence (IF) staining showing endogenous MiD49 and Mff protein levels in wild-type and CLUH KO cells, using anti-MiD49 and anti-Mff antibodies, respectively. e Quantification of IF signals of MiD49 and Mff, normalized to IF signals of a control mitochondrial protein TOM20 (images not shown) in the same cell, using Fiji/ImageJ. f , g Western blots showing Mff and MiD49 protein levels in response to CLUH overexpression or CLUH KO. VDAC was used as a loading control for mitochondrial mass, and actin was used as a housekeeping protein loading control. Western blot experiments were repeated independently 5 times, with representative images shown in ( f ) and statistical analysis shown in ( g ) (mean +- SEM, n = 5). h Quantitative PCR (qPCR) experiments showing Drp1 , Mff , and MiD49 mRNA levels in response to CLUH overexpression or CLUH KO. Total RNAs extracted from HeLa cells of the indicated genotypes were used as templates for the synthesis of cDNAs, which were then used as templates for qPCR. Experiments were repeated in triplicate (mean +- SEM, n = 3). i , j RNA Immunoprecipitation (RIP) experiments show that CLUH binds mRNAs of MiD49 and Mff , but not mRNAs of control genes TOM20 or TBP (encoding the nuclear TATA-binding protein). Experiments were repeated in triplicate (mean +- SEM, n = 3), and representative images are shown in ( j ). k - n ' Puro-PLA was