Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 13685-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCKDHB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TVEDAAQRR QVAHFTFQPD PEPREYGQTQ KMNLFQSVTS ALDNSLAKDP TAVIFGEDVA FGGVFRCTVG LRDKYGKDRV FNTPLCEQGI VGFGIGIAVT GATAIAEIQF ADYIFPAFDQ IVNEAAKYRY RSGDLFNCGS LTIRSPWGCV GHGALYHSQS PEAFFAHCPG IKVVIPRSPF QAKGLLLSCI EDKNPCIFFE PKILYRAAAE EVPIEPYNIP LSQAEVIQEG SDVTLVAWGT QVHVIREVAS MAKEKLGVSC EVIDLRTIIP WDVDTICKSV IKTGRLLISH EAPLTGGFAS EISSTVQEEC FLNLEAPISR VCGYDTPFPH IFEPFYIPDK WKCYDALRKM INY (41-392 aa encoded by BC040139)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 13685-1-AP ( BCKDHB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13685-1-AP (BCKDHB Antibody) at dilution of 1:350 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13685-1-AP (BCKDHB Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13685-1-AP (BCKDHB Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 13685-1-AP ( BCKDHB Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 13685-1-AP ( BCKDHB Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 13685-1-AP) (BCKDHB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
