LS-C55306

antibody from LSBio

Targeting: PPP2R5E B56E, B56epsilon

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C55306

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C55306, RRID:AB_2168150

Product name

PPP2R5E Antibody (aa455-467) LS-C55306

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Porcine, Simian

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody staining (0.5 ug/ml) of mouse brain extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Goat Anti-PPP2R5E Antibody (0.1?/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35? protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody staining (0.5 ug/ml) of mouse brain extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Goat Anti-PPP2R5E Antibody (0.1?/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35? protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.