PA5-85683
antibody from Invitrogen Antibodies
Targeting: WAC
BM-016, FLJ31290, MGC10753, PRO1741, Wwp4
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-85683 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WAC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.35 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WAC in whole cell extracts using WAC polyclonal antibody (Product # PA5-85683) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WAC Polyclonal Antibody detects WAC protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with WAC Polyclonal Antibody (Product # PA5-85683) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WAC Polyclonal Antibody detects WAC protein at nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. WAC Polyclonal Antibody (Product # PA5-85683) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.