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- Antibody Data
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- References [1]
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- Western blot [2]
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- Product number
- NBP2-27509 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal RBM20 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Identification of novel mutations in RBM20 in patients with dilated cardiomyopathy.
Li D, Morales A, Gonzalez-Quintana J, Norton N, Siegfried JD, Hofmeyer M, Hershberger RE
Clinical and translational science 2010 Jun;3(3):90-7
Clinical and translational science 2010 Jun;3(3):90-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM20 Antibody [NBP2-27509] - (1 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM20 Antibody [NBP2-27509] - (0.3ug/ml) staining of HEK293 cell lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RBM20 Antibody [NBP2-27509] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing njuclear and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
