PAB27942

antibody from Abnova Corporation

Targeting: DENND1C FAM31C, FLJ22757

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27942

Provider

Abnova Corporation

Product name

DENND1C polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DENND1C.

Antigen sequence

PLSPEDEGCPWAEEALDSSFLGSGEELDLLSEILD
SLSMGAKSAGSLRPSQSLDCCHRGDLDSCFSLPNI
PRWQPDDKKL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DENND1C polyclonal antibody (Cat # PAB27942) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with DENND1C polyclonal antibody (Cat # PAB27942) at 1-4 ug/mL dilution shows positivity in plasma membrane and focal adhesions.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with DENND1C polyclonal antibody (Cat # PAB27942) shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells at 1:2500-1:5000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)