PA5-60144

antibody from Invitrogen Antibodies

Targeting: DENND1C FAM31C, FLJ22757

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-60144

Provider

Invitrogen Antibodies

Product name

DENND1C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PLSPEDEGCP WAEEALDSSF LGSGEELDLL SEILDSLSMG AKSAGSLRPS QSLDCCHRGD LDSCFSLPNI PRWQPDDKKL

Concentration

0.4 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DENND1C in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a DENND1C Polyclonal Antibody (Product # PA5-60144).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DENND1C in human cell line A-431 shows positivity in plasma membrane & focal adhesion sites. Samples were probed using a DENND1C Polyclonal Antibody (Product # PA5-60144).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DENND1C in human colon using DENND1C Polyclonal Antibody (Product # PA5-60144).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DENND1C in human lymph node shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells. Samples were probed using a DENND1C Polyclonal Antibody (Product # PA5-60144).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DENND1C in human bone marrow using DENND1C Polyclonal Antibody (Product # PA5-60144).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DENND1C in human lymph node and liver tissues using DENND1C Polyclonal Antibody (Product # PA5-60144). Corresponding DENND1C RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DENND1C in human bone marrow, colon, liver and lymph node using DENND1C Polyclonal Antibody (Product # PA5-60144) (A) shows similar protein distribution across tissues to an independent DENND1C Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DENND1C in human liver using DENND1C Polyclonal Antibody (Product # PA5-60144) shows low expression as expected.