Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-60144 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DENND1C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PLSPEDEGCP WAEEALDSSF LGSGEELDLL SEILDSLSMG AKSAGSLRPS QSLDCCHRGD LDSCFSLPNI PRWQPDDKKL Highest antigen sequence identity to the following orthologs: Mouse - 61%, Rat - 61%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DENND1C in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a DENND1C Polyclonal Antibody (Product # PA5-60144).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DENND1C in human cell line A-431 shows positivity in plasma membrane & focal adhesion sites. Samples were probed using a DENND1C Polyclonal Antibody (Product # PA5-60144).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND1C in human colon using DENND1C Polyclonal Antibody (Product # PA5-60144).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND1C in human lymph node shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells. Samples were probed using a DENND1C Polyclonal Antibody (Product # PA5-60144).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND1C in human bone marrow using DENND1C Polyclonal Antibody (Product # PA5-60144).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND1C in human lymph node and liver tissues using DENND1C Polyclonal Antibody (Product # PA5-60144). Corresponding DENND1C RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND1C in human bone marrow, colon, liver and lymph node using DENND1C Polyclonal Antibody (Product # PA5-60144) (A) shows similar protein distribution across tissues to an independent DENND1C Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND1C in human liver using DENND1C Polyclonal Antibody (Product # PA5-60144) shows low expression as expected.