HPA036151

antibody from Atlas Antibodies

Targeting: PRR27 C4orf40

Antibody data

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Validation data

Product number: HPA036151 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA036151, RRID:AB_2674971
Product name: Anti-PRR27
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: RGFPFVPPSRFFSAAAAPAAPPIAAEPAAAAPLTA TPVAAEPAAGAPVAAEPAAEAPVGAEPAAEAPVAA EPAAEAPVGVEPA
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

PRR27 protein structure - HPA036151 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human salivary gland and colon tissues using Anti-PRR27 antibody. Corresponding PRR27 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human colon, lymph node, salivary gland and testis using Anti-PRR27 antibody HPA036151 (A) shows similar protein distribution across tissues to independent antibody HPA036150 (B).
Antibody #2 product nr: HPA036150
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human salivary gland shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis using Anti-PRR27 antibody HPA036151.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node using Anti-PRR27 antibody HPA036151.
Sample type: HUMAN