- GTX100865 - GeneTex SYP antibody

Submitted references Sustained pain hypersensitivity in the stressed colon: Role of mast cell-derived nerve growth factor-mediated enteric synaptic plasticity.

Zhang L, Song J, Bai T, Wang R, Hou X
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2018 Sep;30(9):e13430

Activation of the ileal neuroendocrine tumor cell line P-STS by acetylcholine is amplified by histamine: role of H3R and H4R.

Pfanzagl B, Mechtcheriakova D, Meshcheryakova A, Aberle SW, Pfragner R, Jensen-Jarolim E
Scientific reports 2017 May 2;7(1):1313

REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer.

Chang YT, Lin TP, Campbell M, Pan CC, Lee SH, Lee HC, Yang MH, Kung HJ, Chang PC
Scientific reports 2017 Mar 3;7:42795

I2020T mutant LRRK2 iPSC-derived neurons in the Sagamihara family exhibit increased Tau phosphorylation through the AKT/GSK-3β signaling pathway.

Ohta E, Nihira T, Uchino A, Imaizumi Y, Okada Y, Akamatsu W, Takahashi K, Hayakawa H, Nagai M, Ohyama M, Ryo M, Ogino M, Murayama S, Takashima A, Nishiyama K, Mizuno Y, Mochizuki H, Obata F, Okano H
Human molecular genetics 2015 Sep 1;24(17):4879-900

Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling.

Thomas MG, Saldanha M, Mistry RJ, Dexter DT, Ramsden DB, Parsons RB
Cell death & disease 2013 Jun 13;4:e669

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX100865 diluted at 1:50000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Synaptophysin antibody detects SYP protein by western blot analysis.A. 50 ?g rat brain lysate/extract10% SDS-PAGESynaptophysin antibody (GTX100865) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 12% SDS PAGE GTX100865 diluted at 1:500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Human tissue extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Synaptophysin(GTX100865) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Synaptophysin antibody detects Synaptophysin protein at synaptic vesicles by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using Synaptophysin(GTX100865) antibody at 1:100 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Synaptophysin antibody detects Synaptophysin protein at on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Synaptophysin antibody (GTX100865) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4¢J and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Synaptophysin antibody detects Synaptophysin protein expression by immunohistochemical analysis.Sample: Paraffin-Embedded adult mouse retina. Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).

GTX100865

Antibody data