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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA506067 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506067, RRID:AB_2623575
- Product name
- SYP (Synaptophysin) mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)
- Antibody type
- Monoclonal
- Description
- SYP (Synaptophysin) mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SYP
- Isotype
- IgG
- Antibody clone number
- OTI1G6
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Chronic cigarette smoke exposure drives spiral ganglion neuron loss in mice.
Paquette ST, Dawes RP, Sundar IK, Rahman I, Brown EB, White PM
Scientific reports 2018 Apr 10;8(1):5746
Scientific reports 2018 Apr 10;8(1):5746
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SYP (RC208972, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SYP.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from SH-SY5Y cell line by using anti-SYN monoclonal antibody .(1:500)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (15ug) from mouse brain, rat cerebrum and rat cerebellum by using anti-SYN monoclonal antibody .(1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SYP mouse monoclonal antibody (TA506067) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SYP(RC208972).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-SYP mouse monoclonal antibody (TA506067).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded mouse and rat brain sections within the normal limits using anti-SYN mouse monoclonal antibody.(1:500)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SYP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA506067)
- Validation comment
- IHC
