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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-47307 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLEC5A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse MDL-1 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLEC5A in U937 human histiocytic lymphoma cell line. Samples were incubated in CLEC5A polyclonal antibody (Product # PA5-47307) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for MDL‚1/CLEC5A at approximately 37-40 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CLEC5A in formalin fixed paraffin-embedded sections of human rheumatoid arthritis joint. Samples were incubated in CLEC5A polyclonal antibody (Product # PA5-47307) using a dilution of 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of monocyte colony cells in rheumatoid arthritis joint.
