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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00002550-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002550-M01, RRID:AB_2108165
- Product name
- Mouse Monoclonal GABA-B R1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GABBR1 - gamma-aminobutyric acid (GABA) B receptor, 1
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Limbic encephalitis due to GABAB and AMPA receptor antibodies: a case series.
Conjoint occurrence of GABAB receptor antibodies in Lambert-Eaton myasthenic syndrome with antibodies to the voltage gated calcium channel.
Cortical stimulation causes long-term changes in H-reflexes and spinal motoneuron GABA receptors.
Genome-wide association study reveals multiple nasopharyngeal carcinoma-associated loci within the HLA region at chromosome 6p21.3.
Dogan Onugoren M, Deuretzbacher D, Haensch CA, Hagedorn HJ, Halve S, Isenmann S, Kramme C, Lohner H, Melzer N, Monotti R, Presslauer S, Schäbitz WR, Steffanoni S, Stoeck K, Strittmatter M, Stögbauer F, Trinka E, von Oertzen TJ, Wiendl H, Woermann FG, Bien CG
Journal of neurology, neurosurgery, and psychiatry 2015 Sep;86(9):965-72
Journal of neurology, neurosurgery, and psychiatry 2015 Sep;86(9):965-72
Conjoint occurrence of GABAB receptor antibodies in Lambert-Eaton myasthenic syndrome with antibodies to the voltage gated calcium channel.
Dogan Onugoren M, Rauschka H, Bien CG
Journal of neuroimmunology 2014 Aug 15;273(1-2):115-6
Journal of neuroimmunology 2014 Aug 15;273(1-2):115-6
Cortical stimulation causes long-term changes in H-reflexes and spinal motoneuron GABA receptors.
Wang Y, Chen Y, Chen L, Wolpaw JR, Chen XY
Journal of neurophysiology 2012 Nov;108(10):2668-78
Journal of neurophysiology 2012 Nov;108(10):2668-78
Genome-wide association study reveals multiple nasopharyngeal carcinoma-associated loci within the HLA region at chromosome 6p21.3.
Tse KP, Su WH, Chang KP, Tsang NM, Yu CJ, Tang P, See LC, Hsueh C, Yang ML, Hao SP, Li HY, Wang MH, Liao LP, Chen LC, Lin SR, Jorgensen TJ, Chang YS, Shugart YY
American journal of human genetics 2009 Aug;85(2):194-203
American journal of human genetics 2009 Aug;85(2):194-203
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA-B R1 Antibody (2D7) [H00002550-M01] - Analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi ( Cat # H00002550-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody (M01), clone 2D7 (Cat # H00002550-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA-B R1 Antibody (2D7) [H00002550-M01] - Analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody (M01), clone 2D7.Lane 1: GABBR1 transfected lysate(95 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA-B R1 Antibody (2D7) [H00002550-M01] - GABBR1 monoclonal antibody (M01), clone 2D7 Analysis of GABBR1 expression in IMR-32.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GABA-B R1 Antibody (2D7) [H00002550-M01] - Detection limit for recombinant GST tagged GABBR1 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GABA-B R1 Antibody (2D7) [H00002550-M01] - Analysis of monoclonal antibody to GABBR1 on HeLa cell. Antibody concentration 10 ug/ml.
