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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF7000 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat GABAB R1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects recombinant mouse and rat GABAB R1 in direct ELISAs and Western blots.Detects human and rat GABAB R1 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat GABAB R2 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9Z0U4- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat GABAB R1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat GABAB R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7000) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GABAB R1 at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GABAB R1 in Rat Brain. GABAB R1 was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglia) using Sheep Anti-Mouse/Rat GABAB R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7000) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the cell bodies of dorsal root ganglia neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
