Antibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- AF1226 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Sca-1/Ly6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA28351
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Multicolor quantitative confocal imaging cytometry.
Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress.
Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory properties.
Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms.
Pluripotent stem cells derived from mouse and human white mature adipocytes.
Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy.
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production.
Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors.
Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.
Prominin-1/CD133+ lung epithelial progenitors protect from bleomycin-induced pulmonary fibrosis.
The recruitment of primitive Lin(-) Sca-1(+), CD34(+), c-kit(+) and CD271(+) cells during the early intraperitoneal foreign body reaction.
Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
Identification of bronchioalveolar stem cells in normal lung and lung cancer.
Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46
Nature methods 2018 Jan;15(1):39-46
Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress.
Zhu E, Hu L, Wu H, Piao L, Zhao G, Inoue A, Kim W, Yu C, Xu W, Bando YK, Li X, Lei Y, Hao CN, Takeshita K, Kim WS, Okumura K, Murohara T, Kuzuya M, Cheng XW
Journal of the American Heart Association 2017 Jul 14;6(7)
Journal of the American Heart Association 2017 Jul 14;6(7)
Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory properties.
Daniel JM, Dutzmann J, Brunsch H, Bauersachs J, Braun-Dullaeus R, Sedding DG
International journal of cardiology 2017 Jul 1;238:79-91
International journal of cardiology 2017 Jul 1;238:79-91
Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms.
Golle L, Gerth HU, Beul K, Heitplatz B, Barth P, Fobker M, Pavenstädt H, Di Marco GS, Brand M
Scientific reports 2017 Aug 25;7(1):9444
Scientific reports 2017 Aug 25;7(1):9444
Pluripotent stem cells derived from mouse and human white mature adipocytes.
Jumabay M, Abdmaulen R, Ly A, Cubberly MR, Shahmirian LJ, Heydarkhan-Hagvall S, Dumesic DA, Yao Y, Boström KI
Stem cells translational medicine 2014 Feb;3(2):161-71
Stem cells translational medicine 2014 Feb;3(2):161-71
Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy.
Paxson JA, Gruntman AM, Davis AM, Parkin CM, Ingenito EP, Hoffman AM
Stem cells and development 2013 Dec 15;22(24):3214-25
Stem cells and development 2013 Dec 15;22(24):3214-25
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Driskell RR, Lichtenberger BM, Hoste E, Kretzschmar K, Simons BD, Charalambous M, Ferron SR, Herault Y, Pavlovic G, Ferguson-Smith AC, Watt FM
Nature 2013 Dec 12;504(7479):277-281
Nature 2013 Dec 12;504(7479):277-281
Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production.
Fujita-Sato S, Ito S, Isobe T, Ohyama T, Wakabayashi K, Morishita K, Ando O, Isono F
The Journal of biological chemistry 2011 Sep 9;286(36):31409-17
The Journal of biological chemistry 2011 Sep 9;286(36):31409-17
Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors.
Fujio M, Yamamoto A, Ando Y, Shohara R, Kinoshita K, Kaneko T, Hibi H, Ueda M
Bone 2011 Oct;49(4):693-700
Bone 2011 Oct;49(4):693-700
Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.
Smith BH, Gazda LS, Conn BL, Jain K, Asina S, Levine DM, Parker TS, Laramore MA, Martis PC, Vinerean HV, David EM, Qiu S, Cordon-Cardo C, Hall RD, Gordon BR, Diehl CH, Stenzel KH, Rubin AL
Cancer research 2011 Feb 1;71(3):716-24
Cancer research 2011 Feb 1;71(3):716-24
Prominin-1/CD133+ lung epithelial progenitors protect from bleomycin-induced pulmonary fibrosis.
Germano D, Blyszczuk P, Valaperti A, Kania G, Dirnhofer S, Landmesser U, Lüscher TF, Hunziker L, Zulewski H, Eriksson U
American journal of respiratory and critical care medicine 2009 May 15;179(10):939-49
American journal of respiratory and critical care medicine 2009 May 15;179(10):939-49
The recruitment of primitive Lin(-) Sca-1(+), CD34(+), c-kit(+) and CD271(+) cells during the early intraperitoneal foreign body reaction.
Vranken I, De Visscher G, Lebacq A, Verbeken E, Flameng W
Biomaterials 2008 Mar;29(7):797-808
Biomaterials 2008 Mar;29(7):797-808
Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):3874-81
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):3874-81
Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):3874-81
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):3874-81
A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE
Nature genetics 2008 Jul;40(7):862-70
Nature genetics 2008 Jul;40(7):862-70
Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
Ling TY, Kuo MD, Li CL, Yu AL, Huang YH, Wu TJ, Lin YC, Chen SH, Yu J
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 20;103(25):9530-5
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 20;103(25):9530-5
Identification of bronchioalveolar stem cells in normal lung and lung cancer.
Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T
Cell 2005 Jun 17;121(6):823-35
Cell 2005 Jun 17;121(6):823-35
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Sca-1/Ly6 in Mouse Splenocytes. Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.