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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-34956 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CEP152 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa.
- Concentration
- 1.34 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CEP152 Polyclonal Antibody detects CEP152 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract.5 % SDS-PAGE. CEP152 Polyclonal Antibody (Product # PA5-34956) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CEP152 Polyclonal Antibody detects CEP152 protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: CEP152 protein stained by CEP152 Polyclonal Antibody (Product # PA5-34956) diluted at 1:2,000. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (Product # MA1-25054) diluted at 1:5000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CEP152 was performed in HeLa whole cell extracts using 5 µg of CEP152 Polyclonal Antibody (Product # PA5-34956). Samples were transferred to a membrane and probed with CEP152 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
