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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX128027 - Provider product page
- Provider
- GeneTex
- Product name
- CEP152 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Mosaic-variegated aneuploidy syndrome mutation or haploinsufficiency in Cep57 impairs tumor suppression.
Aziz K, Sieben CJ, Jeganathan KB, Hamada M, Davies BA, Velasco ROF, Rahman N, Katzmann DJ, van Deursen JM
The Journal of clinical investigation 2018 Aug 1;128(8):3517-3534
The Journal of clinical investigation 2018 Aug 1;128(8):3517-3534
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CEP152 antibody detects CEP152 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g HeLa whole cell lysate/extract5 % SDS-PAGECEP152 antibody (GTX128027) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CEP152 antibody detects CEP152 protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min. Green: CEP152 protein stained by CEP152 antibody (GTX128027) diluted at 1:2000. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:5000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of CEP152 protein from HeLa whole cell extracts using 5 £gg of CEP152 antibody (GTX128027).Western blot analysis was performed using CEP152 antibody (GTX128027).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
