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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB8045 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Otolin-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Otolin-1 in ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A6NHN0- Isotype
- IgG
- Antibody clone number
- 839418
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Otolin-1 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells undifferentiated or differentiated to early otic lineage. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Otolin-1 Monoclonal Antibody (Catalog # MAB8045) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Otolin-1 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Otolin-1 in differentiated BG01V Human Embryonic Stem Cells. Otolin-1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to early otic lineage using Mouse Anti-Human Otolin-1 Monoclonal Antibody (Catalog # MAB8045) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
