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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- 10997-2-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLG2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GPLLPGDSA FRLVSAETLR EILQDKELSK EQLVAFLENV LKTSGKLREN LLHGALEHYV NCLDLVNKRL PYGLAQIGVC FHPVFDTKQI RNGVKSIGEK TEASLVWFTP PRTSNQWLDF WLRHRLQWWR KFAMSPSNFS SSDCQDEEGR KGNKLYYNFP WGKELIETLW NLGDHELLHM YPGNVSKLHG RDGRKNVVPC VLSVNGDLDR GMLAYLYDSF QLTENSFTRK KNLHRKVLKL HPCLAPIKVA LDVGRGPTLE LRQVCQGLFN ELLENGISVW PAYLETMQSS LEQLYSKYDE MSILFTVLVT ETTLENGLIH LRSRDTTMKE MMHISKLKDF LIKYISSAKN V (136-485 aa encoded by BC009194)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 10997-2-AP ( POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse colon tissue were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10997-2-AP ( POLG2 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COLO 320 cells using 10997-2-AP (POLG2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COLO 320 cells using 10997-2-AP (POLG2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 10997-2-AP (POLG2 antibody) at dilution of 1:100 (under 10x lens).
