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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-96903 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRICKLE2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QEMEGNLHQL SNPIGYRDLQ SHGRMHQSFD FDGGMAGSKL PGQEGVRIQP MSERTRRRAT SRDDNRRFRP HRSRRSRRSR SDNALHLASE REAISRLKDR PPLRAREDYD QFMRQRSFQE SMGHGSRRDL YGQCPRTVSD LALQNAFGDR WGPYFAEYDW CSTCSSSSES DNEGYFLGEP IPQPARLRYV TSDELLHKYS SYGLPKSSTL GGRGQLHSRK RQKSKNCIIS; Positive Samples: Mouse brain, Rat brain; Cellular Location: Nucleus membrane
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Upregulation of Prickle2 Ameliorates Alzheimer's Disease-Like Pathology in a Transgenic Mouse Model of Alzheimer's Disease.
Sun F, Jiang F, Zhang N, Li H, Tian W, Liu W
Frontiers in cell and developmental biology 2020;8:565020
Frontiers in cell and developmental biology 2020;8:565020
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using PRICKLE2 Polyclonal antibody (Product # PA5-96903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PRICKLE2 in extracts of various cell lines using PRICKLE2 Polyclonal Antibody (Product # PA5-96903) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PRICKLE2 was performed in A549 cells using PRICKLE2 Polyclonal Antibody (Product # PA5-96903).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 1 Prickle2 is downregulated in the brain tissues of 3 x Tg mice. (A) Prickle2 mRNA ( n = 6 per group) in the cortex and the hippocampus isolated from mouse brain. (B) Western blotting for Prickle2 in the cortex and the hippocampus isolated from mouse brain ( n = 6 per group). (C) IHC for Prickle2 in cortex and hippocampus isolated from mouse brain ( n = 6 per group). (D) Western blots in cell lysates from AD mice during aging with antibodies against Prickle2 and GAPDH, as indicated. Blotting intensities are normalized to GAPDH from AD mice at three months old of age (defined as 1.0, n = 6) (A-D) Student's t test. ** p < 0.01; *** p < 0.001; **** p < 0.0001.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 Prickle2 treatment reduces Tau phosphorylation in 3 x Tg mice. (A) Representative IHC images of WT, 3 x Tg and 3 x Tg/Prickle2 treatment mice stained with anti-Tau (phospho S202) antibodies. The proportions of pS202-positive cells were calculated in cortical and hippocampal, respectively ( n = 6 per group). (B) Representative IHC images of WT, 3 x Tg and 3 x Tg/Prickle2 treatment mice stained with anti-Tau (phospho S396) antibodies. The proportions of pS396-positive cells were calculated in cortical and hippocampal, respectively ( n = 6 per group). (C) Western blots detected the phosphorylated Tau using pS202 and pS396 antibodies in the brain tissues from WT, 3 x Tg and 3 x Tg/Prickle2 mice. (D-F) Quantification of the protein levels of phosphorylated Tau by densitometric analyses ( n = 6 per group). (B-D) ANOVA followed by Bonferroni's post hoc test. ** p < 0.01; *** p < 0.001; **** p < 0.0001.
