LS-C817911

antibody from LSBio

Targeting: CNPY2 HP10390, TMEM4, ZSIG9

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C817911

Provider

LSBio

Product name

Cnpy2 / TMEM4 Antibody (Preservative Free) LS-C817911

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CNPY2 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 2 uL anti-CNPY2 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CNPY2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 21 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CNPY2 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 2 uL anti-CNPY2 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CNPY2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 21 kDa.