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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
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- Product number
- NBP1-32578 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32578, RRID:AB_10003964
- Product name
- Rabbit Polyclonal alpha-Actinin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The Power of Human Protective Modifiers: PLS3 and CORO1C Unravel Impaired Endocytosis in Spinal Muscular Atrophy and Rescue SMA Phenotype.
Hosseinibarkooie S, Peters M, Torres-Benito L, Rastetter RH, Hupperich K, Hoffmann A, Mendoza-Ferreira N, Kaczmarek A, Janzen E, Milbradt J, Lamkemeyer T, Rigo F, Bennett CF, Guschlbauer C, Büschges A, Hammerschmidt M, Riessland M, Kye MJ, Clemen CS, Wirth B
American journal of human genetics 2016 Sep 1;99(3):647-665
American journal of human genetics 2016 Sep 1;99(3):647-665
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A:NIH-3T3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody diluted at a dilution of 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-32578] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.
