NBP2-17188

antibody from Novus Biologicals

Targeting: LYN JTK8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-17188

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Lyn Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Lyn Antibody [NBP2-17188] - A. 30 ug Raji whole cell lysate/extract B. 30 ug K562 whole cell lysate/extract C. 30 ug Raji whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody dilution: 1:1000

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Lyn Antibody [NBP2-17188] - IP samples: K562 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of LYN protein by 4 ug LYN antibody 5 % SDS-PAGE The immunoprecipitated LYN protein was detected by LYN antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Lyn Antibody [NBP2-17188] - Human colon carcinoma, using LYN antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min