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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-7274 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-7274, RRID:AB_627897
- Product name
- Anti-LYN
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
Submitted references The caspase-cleaved form of LYN mediates a psoriasis-like inflammatory syndrome in mice.
Integrin-associated Lyn kinase promotes cell survival by suppressing acid sphingomyelinase activity.
Haemophilus ducreyi targets Src family protein tyrosine kinases to inhibit phagocytic signaling.
Marchetti S, Gamas P, Belhacène N, Grosso S, Pradelli LA, Colosetti P, Johansen C, Iversen L, Deckert M, Luciano F, Hofman P, Ortonne N, Khemis A, Mari B, Ortonne JP, Ricci JE, Auberger P
The EMBO journal 2009 Aug 19;28(16):2449-60
The EMBO journal 2009 Aug 19;28(16):2449-60
Integrin-associated Lyn kinase promotes cell survival by suppressing acid sphingomyelinase activity.
Chudakova DA, Zeidan YH, Wheeler BW, Yu J, Novgorodov SA, Kindy MS, Hannun YA, Gudz TI
The Journal of biological chemistry 2008 Oct 24;283(43):28806-16
The Journal of biological chemistry 2008 Oct 24;283(43):28806-16
Haemophilus ducreyi targets Src family protein tyrosine kinases to inhibit phagocytic signaling.
Mock JR, Vakevainen M, Deng K, Latimer JL, Young JA, van Oers NS, Greenberg S, Hansen EJ
Infection and immunity 2005 Dec;73(12):7808-16
Infection and immunity 2005 Dec;73(12):7808-16
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 59
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
