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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX101222 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101222, RRID:AB_10720516
- Product name
- LYN antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Ligand-dependent activation of EphA4 signaling regulates the proteolysis of amyloid precursor protein through a Lyn-mediated pathway.
The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and autoimmunity by inactivating Lck.
Lai WB, Wang BJ, Hu MK, Hsu WM, Her GM, Liao YF
Molecular neurobiology 2014 Apr;49(2):1055-68
Molecular neurobiology 2014 Apr;49(2):1055-68
The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and autoimmunity by inactivating Lck.
Li JP, Yang CY, Chuang HC, Lan JL, Chen DY, Chen YM, Wang X, Chen AJ, Belmont JW, Tan TH
Nature communications 2014 Apr 9;5:3618
Nature communications 2014 Apr 9;5:3618
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LYN antibody detects LYN protein by Western blot analysis.A. 30 ?g Raji whole cell lysate/extractB. 30 ?g K562 whole cell lysate/extractC. 30 ?g Raji whole cell lysate/extract7.5 % SDS-PAGELYN antibody (GTX101222) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN(GTX101222) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LYN antibody immunoprecipitates LYN protein in IP experiments.IP samples: K562 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of LYN protein by 4 £gg LYN antibody (GTX101222)5 % SDS-PAGEThe immunoprecipitated LYN protein was detected by LYN antibody (GTX101222) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LYN(GTX101222) antibody at 1:250 dilution.
