Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN(GTX111584) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
LYN antibody immunoprecipitates LYN protein in IP experiments.IP samples: K562 whole cell extractA. 40 £gg K562 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of LYN protein by 4 £gg LYN antibody (GTX111584)5 % SDS-PAGEThe immunoprecipitated LYN protein was detected by LYN antibody (GTX111584) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]